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Research ArticleArticle

High-Throughput LC-MS/MS Method for Determination of the Alcohol Use Biomarker Phosphatidylethanol in Clinical Samples by Use of a Simple Automated Extraction Procedure—Preanalytical and Analytical Conditions

Anders Isaksson, Lisa Walther, Therese Hansson, Anders Andersson, Joanna Stenton, Anders Blomgren
DOI: 10.1373/jalm.2017.024828 Published December 2017
Anders Isaksson
Department of Laboratory Medicine, Division of Clinical Chemistry and Pharmacology, Lund University, University Hospital, Lund, Sweden.
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  • For correspondence: anders.isaksson@med.lu.se
Lisa Walther
Department of Laboratory Medicine, Division of Clinical Chemistry and Pharmacology, Lund University, University Hospital, Lund, Sweden.
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Therese Hansson
Department of Laboratory Medicine, Division of Clinical Chemistry and Pharmacology, Lund University, University Hospital, Lund, Sweden.
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Anders Andersson
Department of Laboratory Medicine, Division of Clinical Chemistry and Pharmacology, Lund University, University Hospital, Lund, Sweden.
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Joanna Stenton
Department of Laboratory Medicine, Division of Clinical Chemistry and Pharmacology, Lund University, University Hospital, Lund, Sweden.
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Anders Blomgren
Department of Laboratory Medicine, Division of Clinical Chemistry and Pharmacology, Lund University, University Hospital, Lund, Sweden.
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Abstract

Background: Phosphatidylethanol (PEth) is an alcohol use biomarker with higher clinical sensitivity and specificity than commonly used alcohol markers. Since its introduction as a clinical alcohol marker in 2006, the number of samples sent to our laboratory for the determination of PEth has shown a strong annual increase. This has prompted the need to develop a cost-effective and reliable analytical procedure with high capacity.

Methods: An LC-MS/MS method for the determination of PEth 16:0/18:1 with a short turnaround time (3 min) has been evaluated with respect to accuracy, sensitivity, and precision. We compared this method with a previously used HPLC method, as well as a manual and a simplified automated method for sample workup, and investigated potential causes of analytic and preanalytic errors.

Results: The method shows limits of detection and quantification of 0.0075 μmol/L (5.2 ng/mL) and <0.05 μmol/L (<35 ng/mL), respectively. During a 2.1-year period, the method has shown a total CV < 8% for control samples (n = 2808) in the range of 0.10 (70) to 3.5 μmol/L (2461 ng/mL). The simplified automated method for sample preparation works equally well as the manual one. No specific and clinically significant causes of preanalytic errors were found.

Conclusions: This LC-MS/MS method with automated sample workup is well suited for a clinical laboratory with LC-MS/MS experience and has the capability, proven from several years of use, to produce reliable PEth results in a high-volume laboratory (>50000 clinical samples/year).

  • Received July 20, 2017.
  • Accepted October 30, 2017.
  • © 2017 American Association for Clinical Chemistry

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The Journal of Applied Laboratory Medicine: 2 (5)
Vol. 2, Issue 5
March 2018
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High-Throughput LC-MS/MS Method for Determination of the Alcohol Use Biomarker Phosphatidylethanol in Clinical Samples by Use of a Simple Automated Extraction Procedure—Preanalytical and Analytical Conditions
Anders Isaksson, Lisa Walther, Therese Hansson, Anders Andersson, Joanna Stenton, Anders Blomgren
The Journal of Applied Laboratory Medicine Dec 2017, jalm.2017.024828; DOI: 10.1373/jalm.2017.024828
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High-Throughput LC-MS/MS Method for Determination of the Alcohol Use Biomarker Phosphatidylethanol in Clinical Samples by Use of a Simple Automated Extraction Procedure—Preanalytical and Analytical Conditions
Anders Isaksson, Lisa Walther, Therese Hansson, Anders Andersson, Joanna Stenton, Anders Blomgren
The Journal of Applied Laboratory Medicine Dec 2017, jalm.2017.024828; DOI: 10.1373/jalm.2017.024828

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